Highly purified dendritic cells(DC), monocytes and T cells were obtained from peripheral blood by sequential cell fractionation process established recently, Each cell fraction was tested for their susceptibility to primary isolates of HIV-1 from
AIDS
patients. Also, co-culture experiments were performed to investigate HIV-1 transmission in perpheral blood mononuclear cells(PBMC). The results of these experiments show that DC are much more effective than monocytes to transmit HIV-1 to CD4+ T
cells.
Nevertheless these viruses were replicated in CD4+ T cells when they were transmitted by strong antigen presenting cells(APC) like dendritic cells or monocytes. IH strain replicated in DC, butⅡG didn't. Both strain actively replicated in
monocytes.
However, DC were much more effective than monocytes in HIV-1 transmission to CD4+ T cells even in the case ofⅡG transmission HIV-1 transmission or spreading by APC was not inhibited by an anti-HLA-DR monoclonal antibody, suggesting that HIV-1
transmission may not be quite related to classⅡantigen. Cytokines were tested for their involvement in HIV-1 transmission in primary immune cells. IL-2 was the only cytokine required for effective transmission and replication of HIV-1 from DC to
CD4+ T
cells, but IL-2 alone was not enough for monocyte to transmit HIV-1 to CD4+ T cells.
|